Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Article Title: Mechanical stimulations can inhibit local and remote tumor progression by downregulating WISP1
doi: 10.1096/fj.202000713RR
Figure Lengend Snippet: Differential effects of pre- and post-loading urine samples on the expression of WISP1, MMP9, Runx2, and TGFβ in EO771 cells. Of note, pre-S & post-S = pre- & post-step aerobics, and pre-L & post-L = pre- and post-loading. The bar chart is based on 10 pairs of human samples and 10 pairs of mouse samples. (A&B) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to human post-step aerobics urine samples. (C&D) Downregulation of MMP9, RUNX2, TGFβ, and WISP1 in response to murine post-knee loading urine samples.
Article Snippet: We used antibodies against Runx2 (8486s), Snail (3879s), TGFβ (3711s) (Cell Signaling, Danvers, MA, USA), WISP1 (AF1689, R&D_Systems, Minneapolis, MN, USA), MMP9 (sc-393859) (Santa Cruz, Dallas, TX, USA), and β-actin (A5441, Sigma, Saint Louis, MO, USA).
Techniques: Expressing